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Gene Cloning Part 1: The Mechanics of Recombinant DNA: Overview of Recombinant DNA and Gene Cloning Don Lee Department of Agronomy and Horticulture at University of Nebraska-Lincoln, USA Patricia Hain Department of Agronomy and Horticulture at University of Nebraska-Lincoln, USA Lesson Navigation Tips: - Click on 'Animations' button found to the left in order to view the animation which supplements this lesson. You can also click on the animation icon within the text. - Click once on figures to see enlarged versions. - Click once on words in color to bring up their definitions. The process of gene cloning is critical to genetic engineering, gene therapy, gene sequencing and genetic analysis. The basic steps in obtaining a gene from any organism, making recombinant DNA and then cloning this gene in a bacteria are described in this lesson.